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The Danish National Cryo-EM Facility - Aarhus Node

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Facility

Cryo-electron microscopy (cryo-EM) allows high resolution data of protein molecules, other macromolecules, macromolecular complexes or cells to be collected. In 2018 a grant of 30.8 mio DKK (4.7 mio USD) was allocated EMBION from the Ministry of Higher Education and Science – funded from 2015 call of the Danish Roadmap for Research Infrastrucutures. EMBION is a new cryo-EM consortium between Danish Universities (AU host and KU co-host). The National Cryo-EM Facility at AU (iNANO) started up medio 2019 (using existing electron microscopes and facilities). Based on the grant a new high-end electron microscope was purchased: a Titan Krios 300 keV transmission electron microscope with a Bioquantum/K3 detector setup and a Micro-ED system. Installation of the new Titan Krios (Krios2) microscope will finish in Q4 2020 and users can get access to the microscope from Q1 2021. The official inauguration of EMBION is 12th October 2020.

International users please submit application though the iNEXT Discovery programme: https://inext-discovery.eu/



Fully equipped EMlab

An EMlab with the equipment necessary for screening and optimizing samples for negative stain and cryo-EM single particle analysis and other biological EM work is part of the Facility.

Automated data collection

High resolution single particle data can be collected automatically on Titan Krios 1 and 2 using EPU and high resolution tomography data can be collected automatically using SerialEM.

Use of the facility

The Facility offers local AU users, users from other DK research institutions, international academic users (through the iNEXT Discovery program) and industrial users access to state of the art cryo-EM equipment and specialist assistance in a range of areas including single particle analysis and tomography.

Workhorse Tecnai 120 keV microscopes

It is important to screen and optimize samples in order to document that the project is at a stage ready for getting access to the high-end Titan Krios microscopes.



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At embion we have the following

Microscopes



Titan krios 2

Titan Krios G3i (300 keV) - installed 2020

967 Bioquantum energy filter
K3 detector
Ceta-D detector
Single tilt stage
EPU/Tomo5/EPU-D automated data collection software

Titan Krios 300 kV TEM

967 Bioquantum special energy filter with full spectroscopy
K2 Summit detector
Orius 4K detector
US4000 4K detector
Volta Phase Plate
Cs corrector
Double tilt stage
EPU/SerialEM automated data collection software

Titan krios 1
Tecnai Spirit

Tecnai Spirit 120 kV TEM

TWIN lens
Cryo-box
TemCam-F416 (4kx4k) TVIPS CMOS bottom mounted camera
Veleta (2kx2k) CCD side mounted camera
Leginon/Appion automated data collection pipeline

Versa 3D Focused Ion Beam SEM

Cryo-stage for cryo FIB-SEM
Auto Slice and View
High vacuum mode: Everhart-Thornley Detector (ETD)
ESEM(gaseous)mode: Low-vacuum Detector (LVSED) and Gaseous detector (GSED)
vCD, 4-segment backscattered electron detector (BSED)
IR camera, Integrated Infra-Red inspection camera

Versa 3D
Nova NanoSEM

Nova NanoSEM

EDAX EDS system, Energy Dispersive Spectroscopy
SED, Everhart Thornley secondary electron detector.
TLD-SE, in-lens secondary electron detector.
SS-BSED is a solid-state backscattered electron detector.
UHR low vacuum SED (Helix detector)
Low vacuum solid-state BSED (GAD)
IR camera, Integrated Infra-Red inspection camera

By Villy Fink Isaksen

The team

Thomas Vosegaard - iNANO Director

Thomas Vosegaard

iNANO Director

Poul Nissen - DANDRITE Director

Poul Nissen

EMBION Director

Thomas Boesen - Cryo-EM Facility Manager

Thomas Boesen

Cryo-EM Facility Manager

Jesper Lykkegaard Karlsen - Scientific Computing

Jesper L. Karlsen

Scientific Computing

Andreas Boeggild - Senior Applications Specialist

Andreas Boeggild

Senior Applications Specialist

Ramon Liebrechts - EM Technical Manager

Ramon Liebrechts

EM Technical Manager

Pia Bomholt Jensen - EMlab Manager

Pia B. Jensen

EMlab Manager

Taner Drace - Applications Specialist

Taner Drace

Applications Specialist

Acknowledgements

Novo Nordisk Better lives Brainstruc Carlsberg Dandrite CEM Uddannelse og Forskningministeriet Aarhus Universitet og inano